abstract：:The anthelmintic fenbendazole (FBZ) induces nuclear DNA fragmentation (DF) in intestinal cells of Haemonchus contortus. The DNA fragments had 3'-OH, which suggests involvement of a neutral DNase. To identify candidate DNase(s) involved, DNase activity in H. contortus intestine and other worm fractions was characterize...

journal_title：Experimental parasitology

authors： Kwak D,Jasmer DP

更新日期：2003-07-01 00:00:00

abstract：:In rats, CL-Brener clone caused high mortality, severe acute myocarditis, and myositis that subsided completely in surviving animals. Accordingly, no parasite kDNA could be amplified in several organs after 4 months. The monoclonal JG strain caused null mortality, acute predominantly focal myocarditis, discrete and fo...

journal_title：Experimental parasitology

authors： Franco DJ,Vago AR,Chiari E,Meira FC,Galvão LM,Machado CR

更新日期：2003-05-01 00:00:00

abstract：:Cardiac beta-adrenergic receptors' function was studied in the acute phase of Chagas' disease in mice reinfected with Trypanosoma cruzi Tulahuen strain (Tul) and with parasites isolated from an infected patient (SGO-Z12). Genetic characterization of SGO-Z12 isolates demonstrated that it belongs to the zimodeme Z12, on...

journal_title：Experimental parasitology

authors： Bustamante JM,Rivarola HW,Fernández AR,Enders JE,Ricardo F,d'Oro Gloria DL,Palma JA,Paglini-Oliva PA

更新日期：2003-03-01 00:00:00

abstract：:The experiments described here report on the correlation between the ex vivo diffusion of different benzimidazole (BZD) anthelmintics into the cestode parasite Moniezia benedeni, and their octanol-water partition coefficients (P.C.). The characterisation of the drug diffusion process into target parasites is relevant ...

journal_title：Experimental parasitology

authors： Mottier ML,Alvarez LI,Pis MA,Lanusse CE

更新日期：2003-01-01 00:00:00

abstract：:The extracellular metalloproteinases of the insect trypanosomatid Crithidia guilhermei were characterized through the incorporation of different protein substrates (gelatin, casein, haemoglobin, and bovine serum albumin) into SDS-PAGE. Two gelatinases (60 and 80 kDa) showed ability to degrade casein as well and a 67-k...

journal_title：Experimental parasitology

authors： de Melo AC,d'Avila-Levy CM,Branquinha MH,Vermelho AB

更新日期：2002-11-01 00:00:00

abstract：:Resistance and sensitivity to normal human serum (NHS) of Trypanosoma congolense, a parasite believed to cause disease in animals only, were investigated in vivo as well as in vitro. Our results indicate that like Trypanosoma brucei, T. congolense can be grouped into three different phenotypes according to its resista...

journal_title：Experimental parasitology

authors： Xong HV,De Baetselier P,Pays E,Magez S

更新日期：2002-10-01 00:00:00

abstract：:The intestine of Haemonchus contortus is hypersensitive to the effects of the anthelmintic fenbendazole (FBZ). The effects are postulated to stem from disruption of microtubules and interference with apical secretory vesicle transport, followed by release of digestive enzymes into the intestinal cell cytoplasm. Here, ...

journal_title：Experimental parasitology

authors： Shompole S,Yao C,Cheng X,Knox D,Johnson S,Jasmer DP

更新日期：2002-08-01 00:00:00

abstract：:The Gal/GalNAc lectin gene of Entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. We investigated sequence differences in the Gal/GalNAc lectin heavy subunit in three isolates from Bangladesh and one isolate from Georgia, each of which was determined to be genetical...

journal_title：Experimental parasitology

authors： Beck DL,Tanyuksel M,Mackey AJ,Haque R,Trapaidze N,Pearson WR,Loftus B,Petri WA

更新日期：2002-06-01 00:00:00

abstract：:Standard, immunocompetent, inbred strains of mice are non-permissive for infection with the human filarial nematode, Brugia malayi or the closely related Brugia pahangi. This non-permissiveness allows one to address the mechanism(s) that might be used by mammalian hosts to eliminate large, multicellular, metazoan, ext...

journal_title：Experimental parasitology

authors： Rajan TV,Ganley L,Paciorkowski N,Spencer L,Klei TR,Shultz LD

更新日期：2002-04-01 00:00:00

abstract：:A rodent model of malaria, Plasmodium berghei was used to assess the antimalarial potential of dinitroaniline herbicides. Trifluralin, pendimethalin, oryzalin, and benfluralin were all active against P. berghei in vitro at, or close to, submicromolar concentrations, with a rank order of potency similar to that against...

journal_title：Experimental parasitology

authors： Dow GS,Armson A,Boddy MR,Itenge T,McCarthy D,Parkin JE,Thompson RC,Reynoldson JA

更新日期：2002-03-01 00:00:00

abstract：:Hepatocytic metaplasia may be induced in hamsters by carcinogens, and associated with aging, diabetes or chronic pancreatitis. By means of histopathologic and immunohistochemic studies, we observed pancreatic hepatocytes in hamsters infected and reinfected with Trypanosoma cruzi. The change was seen in 18 (19%) out of...

journal_title：Experimental parasitology

authors： dos Santos VM,de Lima MA,Cabrine-Santos M,Marquez Dde S,Reis Md,Pereira Gde A,Lages-Silva E,Ramírez LE

更新日期：2002-02-01 00:00:00

abstract：:In the intestinal tract of fifth instars of the hematophagous reduviid bugs Rhodnius prolixus and Triatoma infestans blood ingestion induced an initial decrease of the concentration of the respective symbiotic bacteria Rhodococcus rhodnii and Nocardia sp. and then within 10 days a 15- or 18-fold increase of the total ...

journal_title：Experimental parasitology

authors： Eichler S,Schaub GA

更新日期：2002-01-01 00:00:00

abstract：:Amebiasis contributes to approximately 50 million cases of life-threatening dysentery worldwide. Comparison of the lectins from Entamoeba histolytica (pathogenic) and Entamoeba dispar (nonpathogenic) was undertaken to elucidate the differential roles of this molecule in invasion versus colonization. Surface lectin was...

journal_title：Experimental parasitology

authors： Pillai DR,Kobayashi S,Kain KC

更新日期：2001-12-01 00:00:00

abstract：:Thirty U.S. Trypanosoma cruzi stocks isolated mainly from wild mammals were characterized by multilocus enzyme electrophoresis at 22 genetic loci and random amplification of polymorphic DNA for 10 primers. Two main phylogenetic clusters, separated by large genetic distances, were discriminated by both methods, corresp...

journal_title：Experimental parasitology

authors： Barnabé C,Yaeger R,Pung O,Tibayrenc M

更新日期：2001-10-01 00:00:00

abstract：:Infection with the protozoan Leishmania donovani in humans is usually subclinical. Parasites probably persist for the life of the host and the low-level infection is controlled by the cellular immune response. To better understand the mechanisms related to the control of infection, we studied the evolution and archite...

journal_title：Experimental parasitology

authors： Melby PC,Tabares A,Restrepo BI,Cardona AE,McGuff HS,Teale JM

更新日期：2001-09-01 00:00:00

abstract：:Phospholipid metabolism of the microsporidian Encephalitozoon cuniculi, an obligate intracellular parasite, has been investigated. Labeled precursor incorporation experiments have shown that phosphatidylserine decarboxylase and phosphatidylethanolamine N-methyltransferase are more active in cells infected by E. cunicu...

journal_title：Experimental parasitology

authors： El Alaoui H,Bata J,Peyret P,Vivarès CP

更新日期：2001-08-01 00:00:00

abstract：:The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distin...

journal_title：Experimental parasitology

authors： Yoder BJ,Goodrum KJ

更新日期：2001-06-01 00:00:00

abstract：:Previous results from our laboratory using pharmacological approaches suggested a role for nitric oxide (NO) in the host defense against the human filarial parasite, Brugia malayi. We sought to determine whether a complementary genetic approach, using mice homozygous for a targeted mutation in the gene encoding induci...

journal_title：Experimental parasitology

authors： Ganley L,Babu S,Rajan TV

更新日期：2001-05-01 00:00:00

abstract：:The contribution of mononuclear phagocytes to host resistance against acute Chagas disease has been studied in vivo in a rat model inoculated with Trypanosoma cruzi, Y strain. Acute T. cruzi infection triggered a dramatic increase (937%) in peripheral blood monocyte number at day 12 of infection. At this point, histol...

journal_title：Experimental parasitology

authors： Melo RC,Machado CR

更新日期：2001-01-01 00:00:00

abstract：:This article describes a sensitive, cheap, and easy method for assaying chemotaxic responses of Leishmania promastigotes. A gradient of the substance to be assayed was produced inside a series of commercially available capillary tubes submerged in a promastigote suspension. After an incubation period, the attractivene...

journal_title：Experimental parasitology

authors： Oliveira JS,Melo MN,Gontijo NF

更新日期：2000-11-01 00:00:00

abstract：:This report describes a molecular method for determining in a first step the generic composition of a nematode community and in a second step, the resistance of each species to benzimidazole (BZ). We first established a polymerase chain reaction (PCR) linked to a restriction fragment length polymorphism strategy using...

journal_title：Experimental parasitology

authors： Silvestre A,Humbert JF

更新日期：2000-08-01 00:00:00

abstract：:The Plasmodium vivax merozoite surface protein-1 (PvMSP-1) has been considered a candidate for a malaria vaccine against erythrocytic stages. PvMSP-1 is immunogenic during natural infections and exhibits antigenic polymorphism. The extent of genetic polymorphism in a region between the so-called interspecies conserved...

journal_title：Experimental parasitology

authors： Gutierrez A,Vicini J,Patarroyo ME,Murillo LA,Patarroyo MA

更新日期：2000-07-01 00:00:00

abstract：:We compared in vitro and in vivo induction of IL-12 (p40) and IFN-gamma by mouse cells stimulated with Toxoplasma gondii, Trypanosoma cruzi, and different species of Leishmania. Spleen cells cultured in vitro with T. cruzi or T. gondii, but not with Leishmania, produced IL-12 (p40) and IFN-gamma. Accordingly, IL-12 (p...

journal_title：Experimental parasitology

authors： Oliveira MA,Santiago HC,Lisboa CR,Ceravollo IP,Trinchieri G,Gazzinelli RT,Vieira LQ

更新日期：2000-06-01 00:00:00

abstract：:Two fasciolicide preparations have been compared in 130 rats experimentally infected with Fasciola hepatica. Parasitological, immunological, and biochemical parameters have been followed to monitor the efficacy of the treatments. While Fascinex (triclabendazole) efficiently cured both male and female rats when adminis...

journal_title：Experimental parasitology

authors： Sibille P,Calléja C,Carreras F,Bigot K,Galtier P,Boulard C

更新日期：2000-04-01 00:00:00

abstract：:Experimental infections of chimpanzees with Onchocerca volvulus and cattle with Onchocerca ochengi provide model systems for research in human onchocerciasis. These infections share many similarities from the standpoint of parasite biology, but little is known about the comparability of immune responses in the two sys...

journal_title：Experimental parasitology

authors： Graham SP,Lustigman S,Trees AJ,Bianco AE

更新日期：2000-03-01 00:00:00

abstract：:We describe the characterization of Tc38, a Trypanosoma cruzi gene coding for a 337-amino-acid protein with a predicted molecular mass of 38 kDa. Tc38 presents similarities to the plant storage vacuolar protein gamma-3-hordein involved in the transport and targeting of prolamins to the vacuole of developing barley end...

journal_title：Experimental parasitology

authors： Robello C,Dallagiovanna B,Castanys S,Gamarro F,Ehrlich R

更新日期：2000-02-01 00:00:00

abstract：:A thrombin (E.C. 3.4.21.5) inhibitor, savignin, was isolated from the salivary glands of Ornithodoros savignyi by a combination of size exclusion, anion-exchange, and reversed-phase chromatography. The inhibitor has a molecular mass of 12,430.4 Da as determined by electrospray mass spectrometry. The behavior of savign...

journal_title：Experimental parasitology

authors： Nienaber J,Gaspar AR,Neitz AW

更新日期：1999-10-01 00:00:00

abstract：:Okuda, K., Esteva, M., Segura, E. L., and Bijovsky, A. T. 1999. The cytostome of Trypanosoma cruzi epimastigotes is associated with the flagellar complex. Experimental Parasitology 92, 223-231. Proliferative forms of Trypanosoma cruzi, amastigotes and epimastigotes, have a cytostome, a specialized structure formed by ...

journal_title：Experimental parasitology

authors： Okuda K,Esteva M,Segura EL,Bijovsy AT

更新日期：1999-08-01 00:00:00

abstract：:Using [(3)H]inulin uptake as a measure of pharyngeal pumping activity, we have investigated and compared the effects of glutamate, ivermectin, and moxidectin on inulin uptake in susceptible and ivermectin-selected Haemonchus contortus. Inulin uptake is inhibited by glutamate, ivermectin, and moxidectin, at biologicall...

journal_title：Experimental parasitology

authors： Paiement JP,Leger C,Ribeiro P,Prichard RK

更新日期：1999-07-01 00:00:00

abstract：:The cysteine-rich C-terminal region of the merozoite surface protein-1, MSP-119, of Plasmodium falciparum has been the most promising vaccine target antigen to date, based on protective immunization studies with recombinant proteins in mice and monkey models. To be further developed as a vaccine candidate, it is essen...

journal_title：Experimental parasitology

authors： Lalitha PV,Malhotra P,Chattopadhyay R,Chauhan VS

更新日期：1999-05-01 00:00:00

abstract：:The expression and activity of topoisomerase I (PfTopoI) has been examined during the intraerythrocytic stages of the Plasmodium falciparum life cycle. The promoter is inactive during the early ring stage and becomes active only during the later trophozoite and schizont stages. The PfTOP1 transcript starts to accumula...

journal_title：Experimental parasitology

authors： Tosh K,Cheesman S,Horrocks P,Kilbey B

更新日期：1999-02-01 00:00:00

abstract：:Two species of macaques, including two Macaca fascicularis from the Philippines, two M. fascicularis from Mauritius, and one Macaca mulatta, were experimentally infected with blood stages of Plasmodium coatneyi and followed during their clinical, parasitological, biological, and immunological evolution. Plasma cytokin...

journal_title：Experimental parasitology

authors： Migot-Nabias F,Ollomo B,Dubreuil G,Morelli A,Domarle O,Nabias R,Georges AJ,Millet P

更新日期：1999-01-01 00:00:00

abstract：:The stability of the gene encoding TSA417, a 72-kDa variant-specific surface protein (VSP) produced by trophozoites of Giardia intestinalis isolate WB-C6, was investigated in isolates of similar (Assemblage A / Group I) or distinct (Assemblage A / Group II) genotype. Using primers specific for the WB-C6 tsa417 gene, D...

journal_title：Experimental parasitology

authors： Ey PL,Darby JM

更新日期：1998-11-01 00:00:00

abstract：:Protective immunity against a Plasmodium falciparum blood infection can be passively transferred by antibodies in humans and in the primate experimental malaria model Saimiri sciureus. We report here the emergence of a novel virulent parasite population after such passive transfer of hyperimmune serum in splenectomize...

journal_title：Experimental parasitology

authors： Morales-Betoulle ME,de la Salmonière YO,Zwetyenga J,Le Scanf C,Jouin H,Michel JC

更新日期：1998-09-01 00:00:00

abstract：:Optimization of desferrioxamine B (DFO) delivery for the treatment of malaria was studied in Plasmodium vinckei infected mice. DFO was administered by three different treatment regimens: (1) multiple subcutaneous injections of free DFO, (2) intraperitoneal infusion of free DFO, or (3) multiple subcutaneous injections ...

journal_title：Experimental parasitology

authors： Postma NS,Hermsen CC,Zuidema J,Eling WM

更新日期：1998-07-01 00:00:00

abstract：:Differences in gene expression between adult and immature Fasciola hepatica (liver fluke) parasites isolated from the mammalian host were investigated using the technique of differential display. For any given primer combination used to produce these displays there were, on average, 22% apparently adult-specific and 1...

journal_title：Experimental parasitology

authors： Reed MB,Spithill TW,Strugnell RA,Panaccio M

更新日期：1998-06-01 00:00:00

abstract：:African trypanosomes exhibit dramatic developmental changes in carbohydrate and energy metabolism, with cytochrome-mediated respiration playing an important role only in the insect stages. The parasites possess three isozymes of malate dehydrogenase, each of which has a different subcellular localization and, likely, ...

journal_title：Experimental parasitology

authors： Anderson SA,Carter V,Parsons M

更新日期：1998-05-01 00:00:00

abstract：:Excystation of Giardia lamblia entails differentiation of dormant cysts into parasitic trophozoites. Despite its importance for infection, this transformation is not understood at the cellular or molecular levels. In these studies, we report that excystation entails detection of environmental stimuli across the tough ...

journal_title：Experimental parasitology

authors： Hetsko ML,McCaffery JM,Svärd SG,Meng TC,Que X,Gillin FD

更新日期：1998-03-01 00:00:00

abstract：:Parasite resistance to antimalarial drugs, particularly chloroquine, is the most disturbing problem of malaria chemotherapy. There is evidence that the codon 86Tyr polymorphism of the Pfmdr1 gene is associated with chloroquine resistance in West African Plasmodium falciparum. The association of this and four other cod...

journal_title：Experimental parasitology

authors： Póvoa MM,Adagu IS,Oliveira SG,Machado RL,Miles MA,Warhurst DC

更新日期：1998-01-01 00:00:00

abstract：:The codon for serine residue 108 of the Plasmodium falciparum dihydrofolate reductase gene was replaced with those for the other 19 amino acids. Except for the Lys108 mutant, which was not expressed, all other substitutions yielded DHFR mutants which were expressed in Escherichia coli as inactive inclusion bodies. Nin...

journal_title：Experimental parasitology

authors： Sirawaraporn W,Yongkiettrakul S,Sirawaraporn R,Yuthavong Y,Santi DV

更新日期：1997-11-01 00:00:00